tube taking care to prevent

ats on the side away from you first); remove the cap from the sterile sample tube taking care to prevent contamination inside the tube; collect sample with tube at 45 degree angle to teat (collect the near New era winter Caps teats first); replace the cap on the tube and dip the teats.Proper sample handling : Refrigerate or keep the sterile milk samples on ice (if plating will occur in less than 24 hours) or freeze the samples (if storing for greater than 24 hours). Plating the sample : Plating the samples requires special bacteriological medium, conditions of a laboratory and personnel with good technique in microbiology. Agitate the milk sample to mix. Plate 0.01 ml milk per each quarter of Blood agar plate using a loop or pipette. For suspected coliforms, plate 0.1 ml milk for each half of a Blood agar plate or plate on MacConkey agar. Incubate at 37 C for 48 hours.Pathogens vs Contaminants : How do you tell if growth on the plate is a pathogen or a contaminant? These criteria assume you are using good technique in collecting the sterile sample and in plating the samples. Greater than or equal to 5 identical colonies from 0.01 ml milk (pure culture) = significant. Greater than or equal to 5 identical colonies from 0.01 ml milk (mixed culture) = significant, but questionable. Less than 5 identical colonies from 0.01 ml milk = a contaminant. Intrepretation : Streptococcus agalactiae and Staphylococcus aureus are new era hats usually significant (representing a causative agent) in any number. Bacillus is usually a contaminant when present in any number. Antibiotic Sensitivity Tests : are often used to determine what antibiotics may affect the bacterium isolated from the udder. However, these take time and additional microbiological techniques. These tests use either disc diffusion or minimal inhibitory concentration assays. The in New era MLB Caps vitro results may not necessarily reflect the in vivo sensitivity of the bacterium.Control of MastitisMastitis Control Awareness of the economic losses associated with mastitis is resulting in a desire for mastitis control programs. Control programs are focused on detection of mastitis (by the above methods), identification of the causative agent(s) and prevention of transmission by removing the source of the agent (milk contaminated fomites, bedding, persistently infected cows, etc.). Knowledge of mammary anatomy and physiology, mammary defense mechanisms, www.neweracap-us.com microbial habitats, microbial virulence factors, milking machine function, and antibiotics/germicides is i